Dissecting polysomes
نویسندگان
چکیده
منابع مشابه
The Configuration of Isolated Polysomes
Behnke's demonstrat ion (l) of helical polysomes in ultrathin sections of fetal rat gut epithelium has raised questions regarding the extent and significance of such structures. Since Behnke's report, helical polysomes have been found in differentiating muscle (2), differentiating pollen mother cells (3), and in the pleuropneumonialike organism A5969 (4). Thus polysome helices have been found i...
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Here we describe a preparative differential centrifugation protocol for the isolation of ribosomes from a crude cell homogenate. The subcellular fraction obtained is enriched in ribosome monomers and polysomes. The protocol has been optimized for the homogenization and collection of the ribosomal fraction from prokaryotic cells, mammalian and plant tissues, reticulocytes, and chloroplasts. The ...
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Recent advances have led to insights into the structure of the bacterial ribosome, but little is known about the 3D organization of ribosomes in the context of translating polysomes. We employed cryoelectron tomography and a template-matching approach to map 70S ribosomes in vitrified bacterial translation extracts and in lysates of active E. coli spheroplasts. In these preparations, polysomal ...
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Polysomes involved in ovalbumin synthesis were identified by the binding of 1251-anti-ovalbumin to hen oviduct polysomes. Techniques were developed for the isolation of undegraded hen oviduct polysomes and for the preparation of 1251-y-globulin free of ribonuclease activity. The distribution of 1251-anti-ovalbumin in the polysome profile is in accordance with the size of the polysomes that are ...
متن کاملIn vitro synthesis of procollagen on polysomes.
The major collagenous products synthesized in a cell-free polysome preparation are pro-alpha1 and pro-alpha2, formed in a ratio of 2:1. They are the precursor forms of alpha1 and alpha2 chains of normal connective-tissue collagen that are also formed in small amounts. A procollagen peptidase activity has been demonstrated in the supernatant fraction that can account for the formation of alpha1 ...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2005
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth0705-488